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Intermediate

Part:BBa_K569002:Design

Designed by: Jose Alfredo Flores   Group: iGEM11_UT_Dallas   (2011-09-27)


RBS+Phototaxis Receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1807
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 349
    Illegal AgeI site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1045
    Illegal BsaI.rc site found at 1324
    Illegal SapI site found at 819
    Illegal SapI.rc site found at 1825


Design Notes

We designed the PCR experiment with the BioBrick standard assembly in mind.


Source

We used the following primers: CCA.TCTAGA.GAGGAGAAATACTAGATGGTGGGAC and CCTGCATAACGCGAAGTAATC to remove the promoter from K317028.

References